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Immunofluorescent staining of human cell line HeLa shows localization to cytosol.
Immunofluorescent staining of human cell line HeLa shows localization to cytosol.
Immunofluorescent staining of human cell line HeLa shows localization to cytosol.

Anti-IRS2 Antibody

HPA054664
Atlas Antibodies
ApplicationsImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetIRS2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-IRS2
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8660
  • Target name
    IRS2
  • Target description
    insulin receptor substrate 2
  • Target synonyms
    insulin receptor substrate 2; IRS-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y4H2
  • Protein Name
    Insulin receptor substrate 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IRS2 using anti-IRS2 antibody (A00805-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRS2 antigen affinity purified polyclonal antibody (Catalog # A00805-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRS2 at approximately 180 kDa. The expected band size for IRS2 is at 137 kDa.
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