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Formalin-fixed and paraffin embedded: rat skeletal muscle labeled with Anti-IRS2 Polyclonal Antibody (bs-0173R), Unconjugated at 1:200followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded: rat skeletal muscle labeled with Anti-IRS2 Polyclonal Antibody (bs-0173R), Unconjugated at 1:200followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded: rat skeletal muscle labeled with Anti-IRS2 Polyclonal Antibody (bs-0173R), Unconjugated at 1:200followed by conjugation to the secondary antibody and DAB staining

IRS-2 Polyclonal Antibody

BS-0173R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetIRS2
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Overview

  • Supplier
    Bioss
  • Product Name
    IRS-2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID8660
  • Target name
    IRS2
  • Target description
    insulin receptor substrate 2
  • Target synonyms
    IRS-2, insulin receptor substrate 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y4H2
  • Protein Name
    Insulin receptor substrate 2
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Wang H, Zhao F, Cai S, et al. MiR-193a regulates chemoresistance of human osteosarcoma cells via repression of IRS2. J Bone Oncol. 2019,17:100241. doi: 10.1016/j.jbo.2019.100241
    Read this paper
  • Wang F, Wang S, Zhang Z, et al. Defective insulin signaling and the protective effects of dimethyldiguanide during follicular development in the ovaries of polycystic ovary syndrome. Mol Med Rep. 2017,16(6):8164-8170. doi: 10.3892/mmr.2017.7678
    Read this paper
  • Ling Q, Xie H, Li J, et al. Donor Graft MicroRNAs: A Newly Identified Player in the Development of New-onset Diabetes After Liver Transplantation. Am J Transplant. 2017,17(1):255-264. doi: 10.1111/ajt.13984
    Read this paper

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Figure 1. Western blot analysis of IRS2 using anti-IRS2 antibody (A00805-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRS2 antigen affinity purified polyclonal antibody (Catalog # A00805-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRS2 at approximately 180 kDa. The expected band size for IRS2 is at 137 kDa.
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