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Immunohistochemical staining of mouse embryo E14 shows moderate to strong nuclear positivity in developing lung.
Immunohistochemical staining of mouse embryo E14 shows moderate to strong nuclear positivity in developing lung.
Immunohistochemical staining of mouse embryo E14 shows moderate to strong nuclear positivity in developing lung.

Anti-IRX1 Antibody

HPA043160
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetIRX1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-IRX1 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID79192
  • Target name
    IRX1
  • Target description
    iroquois homeobox 1
  • Target synonyms
    IRX-5, IRXA1, iroquois-class homeodomain protein IRX-1, homeodomain protein IRXA1, iroquois homeobox protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP78414
  • Protein Name
    Iroquois-class homeodomain protein IRX-1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of IRX1 using anti-IRX1 antibody (A12588-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: rat lung tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse LLC tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRX1 antigen affinity purified polyclonal antibody (Catalog # A12588-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRX1 at approximately 50 kDa. The expected band size for IRX1 is at 50 kDa.
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