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Mouse Cerebrum lysates probed with IRX1 Polyclonal Antibody, Unconjugated (bs-20469R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Mouse Cerebrum lysates probed with IRX1 Polyclonal Antibody, Unconjugated (bs-20469R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Mouse Cerebrum lysates probed with IRX1 Polyclonal Antibody, Unconjugated (bs-20469R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

IRX1 Polyclonal Antibody

BS-20469R
Bioss
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetIRX1
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Overview

  • Supplier
    Bioss
  • Product Name
    IRX1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID79192
  • Target name
    IRX1
  • Target description
    iroquois homeobox 1
  • Target synonyms
    IRX-5, IRXA1, iroquois-class homeodomain protein IRX-1, homeodomain protein IRXA1, iroquois homeobox protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP78414
  • Protein Name
    Iroquois-class homeodomain protein IRX-1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of IRX1 using anti-IRX1 antibody (A12588-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: rat lung tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse LLC tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRX1 antigen affinity purified polyclonal antibody (Catalog # A12588-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRX1 at approximately 50 kDa. The expected band size for IRX1 is at 50 kDa.
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