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Anti-ITCH Antibody

Anti-ITCH Antibody

A99399
Antibodies.com
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-ITCH Antibody
  • Delivery Days Customer
    7
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Rabbit polyclonal antibody to ITCH.
  • Reactivity
    Human, Mouse
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ITCH using anti-ITCH antibody (A00195-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse NIH/3T3 whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human A549 whole cell lysates, Lane 7: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITCH antigen affinity purified polyclonal antibody (Catalog # A00195-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITCH at approximately 103KD. The expected band size for ITCH is at 103KD.
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Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ITCH antibody [GT1248] (GTX02845) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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