_21012823_687.jpg)
WB analysis of wide type (WT) and ITCH knockout (KO) 293T cells using GTX02845 ITCH antibody [GT1248]. Dilution : 1:1000 Loading : 25microg
![WB analysis of various samples using GTX02845 ITCH antibody [GT1248]. Dilution : 1:1000 Loading : 25microg](https://www.genetex.com/upload/website/prouct_img/normal/GTX02845/CutImage_A8624_WB_01_(1073814)_21012823_868.jpg "WB analysis of various samples using GTX02845 ITCH antibody [GT1248]. Dilution : 1:1000 Loading : 25microg")
![WB analysis of various samples using GTX02845 ITCH antibody [GT1248]. Dilution : 1:1000 Loading : 25microg](https://www.genetex.com/upload/website/prouct_img/normal/GTX02845/CutImage_A8624_WB_02_(1073815)_21012823_671.jpg "WB analysis of various samples using GTX02845 ITCH antibody [GT1248]. Dilution : 1:1000 Loading : 25microg")
![Whole cell extract (30 microg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ITCH antibody [GT1248] (GTX02845) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX02845/GTX02845_4000001759_20210122_WB_2_21012823_332.jpg "Whole cell extract (30 microg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ITCH antibody [GT1248] (GTX02845) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 microg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ITCH antibody [GT1248] (GTX02845) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX02845/GTX02845_4000001759_20210122_WB_21012823_954.jpg "Various whole cell extracts (30 microg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ITCH antibody [GT1248] (GTX02845) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
WB analysis of wide type (WT) and ITCH knockout (KO) 293T cells using GTX02845 ITCH antibody [GT1248]. Dilution : 1:1000 Loading : 25microg
ITCH antibody [GT1248] - KO/KD Validated
GTX02845
Overview
- SupplierGeneTex
- Product NameITCH antibody [GT1248] - KO/KD Validated
- Delivery Days Customer10
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT1248
- Concentration1.6 mg/ml
- HostRabbit
- IsotypeIgG
- Scientific DescriptionThis gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]
- ReactivityHuman, Mouse, Rat
- Storage Instruction2°C to 8°C,-20°C
- UNSPSC12352203