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Anti-ITGB5 Antibody

144-02497
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetITGB5
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-ITGB5 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3693
  • Target name
    ITGB5
  • Target description
    integrin subunit beta 5
  • Target synonyms
    integrin beta-5, testis secretory sperm-binding protein Li 217p
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP18084
  • Protein Name
    Integrin beta-5
  • Scientific Description
    ITGB5 Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Integrin beta 5/ITGB5 using anti-Integrin beta 5/ITGB5 antibody (A04201-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: mouse Ana-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin beta 5/ITGB5 antigen affinity purified polyclonal antibody (Catalog # A04201-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin beta 5/ITGB5 at approximately 90KD. The expected band size for Integrin beta 5/ITGB5 is at 90KD.
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