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Western Blot analysis of COS7 cells using Integrin beta5 Polyclonal Antibody
Western Blot analysis of COS7 cells using Integrin beta5 Polyclonal Antibody
Western Blot analysis of COS7 cells using Integrin beta5 Polyclonal Antibody

ITGB5 Antibody

CSB-PA003052
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat
TargetITGB5
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Overview

  • Supplier
    Cusabio
  • Product Name
    ITGB5 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3693
  • Target name
    ITGB5
  • Target description
    integrin subunit beta 5
  • Target synonyms
    integrin beta-5; testis secretory sperm-binding protein Li 217p
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP18084
  • Protein Name
    Integrin beta-5
  • Reactivity
    Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Integrin beta 5/ITGB5 using anti-Integrin beta 5/ITGB5 antibody (A04201-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: mouse Ana-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin beta 5/ITGB5 antigen affinity purified polyclonal antibody (Catalog # A04201-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin beta 5/ITGB5 at approximately 90KD. The expected band size for Integrin beta 5/ITGB5 is at 90KD.
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