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Anti-JHDM1a/FBXL11 (Center) Antibody

102-20485
RayBiotech
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetKDM2A
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-JHDM1a/FBXL11 (Center) Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    RB14392
  • Gene ID22992
  • Target name
    KDM2A
  • Target description
    lysine demethylase 2A
  • Target synonyms
    CXXC8, FBL11, FBL7, FBXL11, JHDM1A, LILINA, lysine-specific demethylase 2A, CXXC-type zinc finger protein 8, F-box and leucine-rich repeat protein 11, F-box/LRR-repeat protein 11, [Histone-H3]-lysine-36 demethylase 1A, jmjC domain-containing histone demethylation protein 1A, jumonji C domain-containing histone demethylase 1A, lysine (K)-specific demethylase 2A
  • Host
    Rabbit
  • Protein IDP59997
  • Protein Name
    Lysine-specific demethylase 2A
  • Scientific Description
    Rabbit Anti-JHDM1a/FBXL11 (Center) Antibody, 400 microl
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FBXL11 using anti-FBXL11 antibody (A03027-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse liver tissue lysates, Lane 2: human A431 cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FBXL11 antigen affinity purified polyclonal antibody (Catalog # A03027-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FBXL11 at approximately 150KD. The expected band size for FBXL11 is at 133KD.
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