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Western blot analysis of lysates from A549 and COS7 cells, using K6PP Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from A549 and COS7 cells, using K6PP Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from A549 and COS7 cells, using K6PP Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-K6PP Antibody

A30471
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat
TargetPFKP
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-K6PP Antibody
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Gene ID5214
  • Target name
    PFKP
  • Target description
    phosphofructokinase, platelet
  • Target synonyms
    ATP-PFK, PFK-C, PFK-P, PFKF, ATP-dependent 6-phosphofructokinase, platelet type, 6-phosphofructokinase type C, 6-phosphofructokinase, platelet type, phosphofructo-1-kinase isozyme C, phosphofructokinase 1, phosphohexokinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ01813
  • Protein Name
    ATP-dependent 6-phosphofructokinase, platelet type
  • Scientific Description
    Boster Bio Anti-K6PP Antibody (Catalog# A30471). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat, Monkey.
  • Reactivity
    Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of PFKP using anti-PFKP antibody (A07337-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PFKP antigen affinity purified polyclonal antibody (Catalog # A07337-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PFKP at approximately 86 kDa. The expected band size for PFKP is at 86, 80-86 kDa.
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