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Immunohistochemical staining of human cerebral cortex shows strong membranous positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows strong membranous positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows strong membranous positivity in neurons.

Anti-KCNB1 Antibody

HPA042434
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetKCNB1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-KCNB1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3745
  • Target name
    KCNB1
  • Target description
    potassium voltage-gated channel subfamily B member 1
  • Target synonyms
    DEE26; delayed rectifier potassium channel 1; DRK1; Kv2.1; potassium voltage-gated channel subfamily B member 1; potassium voltage-gated channel, Shab-related subfamily, member 1; voltage-gated potassium channel subunit Kv2.1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14721
  • Protein Name
    Potassium voltage-gated channel subfamily B member 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Kv2.1 using anti-Kv2.1 antibody (PB9111). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kv2.1 antigen affinity purified polyclonal antibody (Catalog # PB9111) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Kv2.1 at approximately 96 kDa. The expected band size for Kv2.1 is at 96 kDa.
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WB analysis of extracts from K562 cells treated with TNF using GTX55387 Kv2.1 (phospho Ser805) antibody. Left : Primary antibody Right : Primary antibody pre-incubated with the antigen specific peptide
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