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ProductsBack/Goat anti-KCNB1 / DRK1

Goat anti-KCNB1 / DRK1

EB08061
Everest Biotech
ApplicationsELISA
Product group Antibodies
TargetKCNB1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-KCNB1 / DRK1 Antibody
  • Delivery Days Customer
    5
  • Applications
    ELISA
  • Applications Supplier
    Pep-ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID3745
  • Target name
    KCNB1
  • Target description
    potassium voltage-gated channel subfamily B member 1
  • Target synonyms
    DEE26; delayed rectifier potassium channel 1; DRK1; Kv2.1; potassium voltage-gated channel subfamily B member 1; potassium voltage-gated channel, Shab-related subfamily, member 1; voltage-gated potassium channel subunit Kv2.1
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_004966.1. GeneIDs all Nonhuman: 16500 (mouse); 25736 (rat);. Purification: Antigen affinity purified. Names and symbols: KCNB1; potassium voltage-gated channel, Shab-related subfamily, member 1; DRK1; KV2.1; h-DRK1; delayed rectifier potassium channel Kv2.1; h-DRK1 K channel; potassium channel protein DRK1; potassium voltage-gated channel subfamily B member 1; voltage-gated
  • Reactivity Supplier
    Human, Mouse, Dog, Cow, Pig
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Kv2.1 using anti-Kv2.1 antibody (PB9111). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kv2.1 antigen affinity purified polyclonal antibody (Catalog # PB9111) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Kv2.1 at approximately 96 kDa. The expected band size for Kv2.1 is at 96 kDa.
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WB analysis of extracts from K562 cells treated with TNF using GTX55387 Kv2.1 (phospho Ser805) antibody. Left : Primary antibody Right : Primary antibody pre-incubated with the antigen specific peptide
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