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Anti-KDM5B Antibody

Anti-KDM5B Antibody

A38071
Antibodies.com
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-KDM5B Antibody
  • Delivery Days Customer
    7
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    2.7 mg/ml
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Scientific Description
    Rabbit polyclonal antibody to KDM5B
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of KDM5B using anti-KDM5B antibody (PB10073). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: monkey COS-7 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KDM5B antigen affinity purified polyclonal antibody (Catalog # PB10073) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KDM5B at approximately 180KD. The expected band size for KDM5B is at 180KD.
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