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Immunohistochemical staining of human bone marrow shows distinct cytoplasmic positivity in subsets of hematopoietic cells.
Immunohistochemical staining of human bone marrow shows distinct cytoplasmic positivity in subsets of hematopoietic cells.
Immunohistochemical staining of human bone marrow shows distinct cytoplasmic positivity in subsets of hematopoietic cells.

Anti-KDM6A Antibody

HPA001165
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetKDM6A
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-KDM6A
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7403
  • Target name
    KDM6A
  • Target description
    lysine demethylase 6A
  • Target synonyms
    KABUK2, UTX, bA386N14.2, lysine-specific demethylase 6A, [histone H3]-trimethyl-L-lysine(27) demethylase 6A, bA386N14.2 (ubiquitously transcribed X chromosome tetratricopeptide repeat protein (UTX)), histone demethylase UTX, lysine (K)-specific demethylase 6A, ubiquitously transcribed tetratricopeptide repeat protein X-linked, ubiquitously-transcribed TPR gene on the X chromosome
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15550
  • Protein Name
    Lysine-specific demethylase 6A
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of UTX/KDM6A using anti-UTX/KDM6A antibody (A01286-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HEK293 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UTX/KDM6A antigen affinity purified polyclonal antibody (Catalog # A01286-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UTX/KDM6A at approximately 154 kDa. The expected band size for UTX/KDM6A is at 154 kDa.
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