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Anti-KIAA1429 Antibody

144-64201
RayBiotech
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetVIRMA
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-KIAA1429 Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Conjugate
    Unconjugated
  • Gene ID25962
  • Target name
    VIRMA
  • Target description
    vir like m6A methyltransferase associated
  • Target synonyms
    KIAA1429, MSTP054, fSAP121, protein virilizer homolog, functional spliceosome-associated protein 121, virilizer homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ69YN4
  • Protein Name
    Protein virilizer homolog
  • Scientific Description
    KIAA1429 pAb
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of KIAA1429/VIRMA using anti-KIAA1429/VIRMA antibody (A32283-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human SK-OV-3 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIAA1429/VIRMA antigen affinity purified polyclonal antibody (Catalog # A32283-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KIAA1429/VIRMA at approximately 202 kDa. The expected band size for KIAA1429/VIRMA is at 202 kDa.
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