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Immunohistochemical staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.

Anti-KIAA1429 Antibody

HPA031530
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetVIRMA
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-KIAA1429 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID25962
  • Target name
    VIRMA
  • Target description
    vir like m6A methyltransferase associated
  • Target synonyms
    KIAA1429, MSTP054, fSAP121, protein virilizer homolog, functional spliceosome-associated protein 121, virilizer homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ69YN4
  • Protein Name
    Protein virilizer homolog
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of KIAA1429/VIRMA using anti-KIAA1429/VIRMA antibody (A32283-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human SK-OV-3 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIAA1429/VIRMA antigen affinity purified polyclonal antibody (Catalog # A32283-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KIAA1429/VIRMA at approximately 202 kDa. The expected band size for KIAA1429/VIRMA is at 202 kDa.
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