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Immunofluorescent staining of human cell line Hep G2 shows localization to nucleus.
Immunofluorescent staining of human cell line Hep G2 shows localization to nucleus.
Immunofluorescent staining of human cell line Hep G2 shows localization to nucleus.

Anti-KIN Antibody

HPA038700
Atlas Antibodies
ApplicationsChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetKIN
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-KIN
  • Delivery Days Customer
    4
  • Applications
    ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID22944
  • Target name
    KIN
  • Target description
    Kin17 DNA and RNA binding protein
  • Target synonyms
    binding to curved DNA; BTCD; DNA/RNA-binding protein KIN17; KIN, antigenic determinant of recA protein homolog; KIN17; Rts2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60870
  • Protein Name
    DNA/RNA-binding protein KIN17
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of KIN using anti-KIN antibody (PB9607). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: rat NRK whole cell lysates, Lane 7: mouse HEPA1-6 whole cell lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIN antigen affinity purified polyclonal antibody (Catalog # PB9607) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KIN at approximately 45 kDa. The expected band size for KIN is at 45 kDa.
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