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Western blot analysis of KLF10 expression in (1) HepG2 cell lysate; (2) NIH/3T3 cell lysate.
Western blot analysis of KLF10 expression in (1) HepG2 cell lysate; (2) NIH/3T3 cell lysate.
Western blot analysis of KLF10 expression in (1) HepG2 cell lysate; (2) NIH/3T3 cell lysate.

Anti-KLF10 Rabbit Monoclonal Antibody

M03419
Boster Bio
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetKLF10
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-KLF10 Rabbit Monoclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    22K32
  • Gene ID7071
  • Target name
    KLF10
  • Target description
    KLF transcription factor 10
  • Target synonyms
    EGR-alpha, EGRA, TIEG, TIEG1, Krueppel-like factor 10, Kruppel like factor 10, TGFB-inducible early growth response protein 1, early growth response-alpha, transforming growth factor-beta-inducible early growth response protein 1, zinc finger transcription factor TIEG
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13118
  • Protein Name
    Krueppel-like factor 10
  • Scientific Description
    Boster Bio Anti-KLF10 Rabbit Monoclonal Antibody catalog # M03419. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Zheng L, Lu H, Li H, et al. KLF10 is upregulated in osteoarthritis and inhibits chondrocyte proliferation and migration by upregulating Acvr1 and suppressing inhbb expression. Acta Histochem. 2020,122(3):151528. doi: 10.1016/j.acthis.2020.151528
    Read this paper

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MSDS

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Figure 1. Western blot analysis of KLF10 using anti-KLF10 antibody (A03419-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KLF10 antigen affinity purified polyclonal antibody (Catalog # A03419-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KLF10 at approximately 55 kDa. The expected band size for KLF10 is at 53 kDa.
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