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Immunohistochemical staining of human stomach shows moderate to strong nuclear immunoreactivity in glandular cells.
Immunohistochemical staining of human stomach shows moderate to strong nuclear immunoreactivity in glandular cells.
Immunohistochemical staining of human stomach shows moderate to strong nuclear immunoreactivity in glandular cells.

Anti-KLF4 Antibody

AMAB91389
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetKLF4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-KLF4
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL5785
  • Conjugate
    Unconjugated
  • Gene ID9314
  • Target name
    KLF4
  • Target description
    KLF transcription factor 4
  • Target synonyms
    EZF, GKLF, Krueppel-like factor 4, Kruppel like factor 4, Kruppel-like factor 4 (gut), endothelial Kruppel-like zinc finger protein, epithelial zinc finger protein EZF, gut Kruppel-like factor, gut-enriched krueppel-like factor
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDO43474
  • Protein Name
    Krueppel-like factor 4
  • Scientific Description
    Synthetic Peptide
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of KLF4 using anti-KLF4 antibody (A00120-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KLF4 antigen affinity purified polyclonal antibody (Catalog # A00120-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KLF4 at approximately 60 kDa. The expected band size for KLF4 is at 60 kDa.
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