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Western blot All lanes: Krueppel-like factor 4 antibody at 5ug/ml + 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 55, 52, 46, 13, 7 kDa Observed band size: 55 kDa
Western blot All lanes: Krueppel-like factor 4 antibody at 5ug/ml + 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 55, 52, 46, 13, 7 kDa Observed band size: 55 kDa
Western blot All lanes: Krueppel-like factor 4 antibody at 5ug/ml + 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 55, 52, 46, 13, 7 kDa Observed band size: 55 kDa

KLF4 Antibody

CSB-PA012394DSR2HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetKLF4
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Overview

  • Supplier
    Cusabio
  • Product Name
    KLF4 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9314
  • Target name
    KLF4
  • Target description
    Kruppel like factor 4
  • Target synonyms
    endothelial Kruppel-like zinc finger protein; epithelial zinc finger protein EZF; EZF; GKLF; gut Kruppel-like factor; gut-enriched krueppel-like factor; Krueppel-like factor 4; Kruppel-like factor 4 (gut)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43474
  • Protein Name
    Krueppel-like factor 4
  • Scientific Description
    Transcription factor; can act both as activator and as repressor. Binds the 5-CACCC-3 core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of KLF4 using anti-KLF4 antibody (A00120-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KLF4 antigen affinity purified polyclonal antibody (Catalog # A00120-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KLF4 at approximately 60 kDa. The expected band size for KLF4 is at 60 kDa.
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