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Anti-KLK1 Antibody

144-01807
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetKLK1
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-KLK1 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3816
  • Target name
    KLK1
  • Target description
    kallikrein 1
  • Target synonyms
    KLKR, Klk6, hK1, kallikrein-1, glandular kallikrein 1, kallikrein 1, renal/pancreas/salivary, kallikrein serine protease 1, kidney/pancreas/salivary gland kallikrein, tissue kallikrein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP06870
  • Protein Name
    Kallikrein-1
  • Scientific Description
    KLK1 Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Kallikrein 1 using anti-Kallikrein 1 antibody (A02789). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human KLK1 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kallikrein 1 antigen affinity purified polyclonal antibody (Catalog # A02789) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Kallikrein 1 at approximately 32KD. The expected band size for Kallikrein 1 is at 29KD.
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