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Western Blot analysis of MCF7 cells using KLK1 Polyclonal Antibody.
Western Blot analysis of MCF7 cells using KLK1 Polyclonal Antibody.
Western Blot analysis of MCF7 cells using KLK1 Polyclonal Antibody.

KLK1 Antibody

CSB-PA005798
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetKLK1
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Overview

  • Supplier
    Cusabio
  • Product Name
    KLK1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3816
  • Target name
    KLK1
  • Target description
    kallikrein 1
  • Target synonyms
    KLKR, Klk6, hK1, kallikrein-1, glandular kallikrein 1, kallikrein 1, renal/pancreas/salivary, kallikrein serine protease 1, kidney/pancreas/salivary gland kallikrein, tissue kallikrein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP06870
  • Protein Name
    Kallikrein-1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Kallikrein 1 using anti-Kallikrein 1 antibody (A02789). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human KLK1 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kallikrein 1 antigen affinity purified polyclonal antibody (Catalog # A02789) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Kallikrein 1 at approximately 32KD. The expected band size for Kallikrein 1 is at 29KD.
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