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Immunohistochemical staining of human testis shows moderate granular cytoplasmic positivity in Leydig cells.
Immunohistochemical staining of human testis shows moderate granular cytoplasmic positivity in Leydig cells.
Immunohistochemical staining of human testis shows moderate granular cytoplasmic positivity in Leydig cells.

Anti-LACTB Antibody

HPA036362
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetLACTB
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-LACTB
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID114294
  • Target name
    LACTB
  • Target description
    lactamase beta
  • Target synonyms
    G24; mitochondrial 39S ribosomal protein L56; MRPL56; serine beta-lactamase-like protein LACTB, mitochondrial; serine beta-lactamase-like protein, mitochondrial
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP83111
  • Protein Name
    Serine beta-lactamase-like protein LACTB, mitochondrial
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LACTB using anti-LACTB antibody (A13145-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LACTB antigen affinity purified polyclonal antibody (Catalog # A13145-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LACTB at approximately 54 kDa. The expected band size for LACTB is at 54 kDa.
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