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ProductsBack/beta Lactamase antibody [N1C1]

beta Lactamase antibody [N1C1]

GTX112574
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetLACTB
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Overview

  • Supplier
    GeneTex
  • Product Name
    beta Lactamase antibody [N1C1]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID114294
  • Target name
    LACTB
  • Target description
    lactamase beta
  • Target synonyms
    G24; mitochondrial 39S ribosomal protein L56; MRPL56; serine beta-lactamase-like protein LACTB, mitochondrial; serine beta-lactamase-like protein, mitochondrial
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP83111
  • Protein Name
    Serine beta-lactamase-like protein LACTB, mitochondrial
  • Scientific Description
    This gene encodes a protein from the large 39S subunit of the mitochondrial ribosome (mitoribosome). The encoded protein has some sequence similarity to prokaryotic beta-lactamases but most of the residues that are responsible for the beta-lactamase activity are not conserved between the two proteins. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LACTB using anti-LACTB antibody (A13145-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LACTB antigen affinity purified polyclonal antibody (Catalog # A13145-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LACTB at approximately 54 kDa. The expected band size for LACTB is at 54 kDa.
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