Anti-LAG-3 [ADI-31798]

Absolute Antibody

Anti-LAG-3 [ADI-31798]

7

This antibody binds human LAG-3. The human LAG-3 is a cell surface molecule with diverse biologic effects on T cell function. It is an immune checkpoint receptor on activated T-cells.

This antibody can bind human LAG-3 in an ELISA. This antibody binds human LAG-3 with an affinity of Kd= 9.73 X 10^(-10) nM. The binding specificity of this antibody to human LAG-3 expressed on the surface of HEK293 and activated CD4+ T cells was done using flow cytometry. This antibody binds hLAG-3 expressed on the surface of HEK293 cells with an EC50 of 0.201 nM and activated CD4+ T cells with an EC50 of 0.00595 nM. This antibody can also effectively block the binding of LAG-3 and its ligand MHCII (HLA-DR) with an IC50 of 4.701 nM. The tumor suppressive effects of this antibody was studied in A375 human PBMC model (CN112166125).

Flow Cytometry, ELISA

FC; ELISA

Research Use Only

Monoclonal

ADI-31798

LAG3

lymphocyte activating 3

CD223; lymphocyte activation gene 3 protein; lymphocyte-activation gene 3

Human

IgG1

Lymphocyte activation gene 3 protein

This reformatted human antibody was made using the variable domain sequences of the original Human IgG4 format for improved compatibility with existing reagents assays and techniques.

Human

Human

The original antibody was isolated from a yeast display library by two rounds of screening against human LAG-3.

-20°C,2°C to 8°C

12352203