Anti-LAG-3 [ADI-31798]

Absolute Antibody

Anti-LAG-3 [ADI-31798]

9

This antibody binds human LAG-3. The human LAG-3 is a cell surface molecule with diverse biologic effects on T cell function. It is an immune checkpoint receptor on activated T-cells.

This antibody can bind human LAG-3 in an ELISA. This antibody binds human LAG-3 with an affinity of Kd= 9.73 X 10^(-10) nM. The binding specificity of this antibody to human LAG-3 expressed on the surface of HEK293 and activated CD4+ T cells was done using flow cytometry. This antibody binds hLAG-3 expressed on the surface of HEK293 cells with an EC50 of 0.201 nM and activated CD4+ T cells with an EC50 of 0.00595 nM. This antibody can also effectively block the binding of LAG-3 and its ligand MHCII (HLA-DR) with an IC50 of 4.701 nM. The tumor suppressive effects of this antibody was studied in A375 human PBMC model (CN112166125).

Flow Cytometry, ELISA

Research Use Only

Monoclonal

ADI-31798

LAG3

lymphocyte activating 3

CD223; lymphocyte activation gene 3 protein; lymphocyte-activation gene 3

Rabbit

IgG

Lymphocyte activation gene 3 protein

This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG4 format for improved compatibility with existing reagents assays and techniques.

Human

-20°C,2°C to 8°C

12352203