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Immunohistochemical staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.
Immunohistochemical staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.
Immunohistochemical staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.

Anti-LAGE3 Antibody

HPA036122
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetLAGE3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-LAGE3 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8270
  • Target name
    LAGE3
  • Target description
    L antigen family member 3
  • Target synonyms
    CVG5, DXS9879E, DXS9951E, ESO3, GAMOS2, ITBA2, Pcc1, EKC/KEOPS complex subunit LAGE3, protein ESO-3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14657
  • Protein Name
    EKC/KEOPS complex subunit LAGE3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of LAGE3 using anti-LAGE3 antibody (A15245-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LAGE3 antigen affinity purified polyclonal antibody (Catalog # A15245-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LAGE3 at approximately 17 kDa. The expected band size for LAGE3 is at 15 kDa.
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