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LAGE3 Antibody (N-Terminus)

LS-C201798
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetLAGE3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    LAGE3 Antibody (N-Terminus)
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Applications Supplier
    WB (1 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8270
  • Target name
    LAGE3
  • Target description
    L antigen family member 3
  • Target synonyms
    CVG5, DXS9879E, DXS9951E, ESO3, GAMOS2, ITBA2, Pcc1, EKC/KEOPS complex subunit LAGE3, protein ESO-3
  • Host
    Rabbit
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LAGE3 using anti-LAGE3 antibody (A15245-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LAGE3 antigen affinity purified polyclonal antibody (Catalog # A15245-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LAGE3 at approximately 17 kDa. The expected band size for LAGE3 is at 15 kDa.
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