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Immunohistochemical staining of human rectum shows strong immunoreactivity in basement membrane of glandular epithelium.
Immunohistochemical staining of human rectum shows strong immunoreactivity in basement membrane of glandular epithelium.
Immunohistochemical staining of human rectum shows strong immunoreactivity in basement membrane of glandular epithelium.

Anti-LAMC1 Antibody

AMAB91136
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetLAMC1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-LAMC1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL3195
  • Conjugate
    Unconjugated
  • Gene ID3915
  • Target name
    LAMC1
  • Target description
    laminin subunit gamma 1
  • Target synonyms
    LAMB2; laminin B2 chain; laminin subunit gamma-1; laminin, gamma 1 (formerly LAMB2); laminin-10 subunit gamma; laminin-11 subunit gamma; laminin-2 subunit gamma; laminin-3 subunit gamma; laminin-4 subunit gamma; laminin-6 subunit gamma; laminin-7 subunit gamma; laminin-8 subunit gamma; laminin-9 subunit gamma; S-LAM gamma; S-laminin subunit gamma
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDP11047
  • Protein Name
    Laminin subunit gamma-1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Laminin Gamma 1/LAMC1 using anti-Laminin Gamma 1/LAMC1 antibody (A03522-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: rat lung tissue lysates, Lane 3: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Laminin Gamma 1/LAMC1 antigen affinity purified polyclonal antibody (Catalog # A03522-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Laminin Gamma 1/LAMC1 at approximately 200 kDa. The expected band size for Laminin Gamma 1/LAMC1 is at 178 kDa.
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