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Anti-Laminin [A5]

Ab02770-10.0
Absolute Antibody
ApplicationsImmunoFluorescence, ImmunoPrecipitation, ImmunoHistoChemistry
Product group Antibodies
TargetLAMC1
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Laminin [A5]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This original rat IgG2a clone can be used to detect and analyze the presence of laminin. The specificity towards this protein has been confirmed using immunoprecipitation (Ljubimov et al., 1986; PMID: 3522258). The location of the laminin was confirmed to be in the basment membrane by means of immunofluorescence using this antibody (Eriksdotter-Nielson et al., 1986; PMID: 3537540).
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, ImmunoHistoChemistry
  • Applications Supplier
    IP; IHC; IF
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    A5
  • Gene ID3915
  • Target name
    LAMC1
  • Target description
    laminin subunit gamma 1
  • Target synonyms
    LAMB2; laminin B2 chain; laminin subunit gamma-1; laminin, gamma 1 (formerly LAMB2); laminin-10 subunit gamma; laminin-11 subunit gamma; laminin-2 subunit gamma; laminin-3 subunit gamma; laminin-4 subunit gamma; laminin-6 subunit gamma; laminin-7 subunit gamma; laminin-8 subunit gamma; laminin-9 subunit gamma; S-LAM gamma; S-laminin subunit gamma
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDP11047
  • Protein Name
    Laminin subunit gamma-1
  • Scientific Description
    This chimeric human antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    Human, Mouse, Bovine
  • Reactivity Supplier Note
    The original rat IgG2a version of this antibody was raised by immunizing fisher rats with high molecular mass material from the Engelbreth-Holm-Swarm (EHS) tumor matrix containing laminin, entactin and HSPG, and fusing the spleen cells of these rats with mouse myeloma X63 Ag8.653 cells.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Laminin Gamma 1/LAMC1 using anti-Laminin Gamma 1/LAMC1 antibody (A03522-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: rat lung tissue lysates, Lane 3: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Laminin Gamma 1/LAMC1 antigen affinity purified polyclonal antibody (Catalog # A03522-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Laminin Gamma 1/LAMC1 at approximately 200 kDa. The expected band size for Laminin Gamma 1/LAMC1 is at 178 kDa.
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