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Immunohistochemical staining of human prostate shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human prostate shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human prostate shows strong granular cytoplasmic positivity in glandular cells.

Anti-LAMTOR1 Antibody

HPA002997
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetLAMTOR1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-LAMTOR1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55004
  • Target name
    LAMTOR1
  • Target description
    late endosomal/lysosomal adaptor, MAPK and MTOR activator 1
  • Target synonyms
    C11orf59, PDRO, Ragulator1, p18, p27RF-Rho, ragulator complex protein LAMTOR1, RhoA activator C11orf59, late endosomal/lysosomal adaptor and MAPK and MTOR activator 1, lipid raft adaptor protein p18, p27Kip1-releasing factor from RhoA, p27kip1 releasing factor from RhoA, protein associated with DRMs and endosomes, ragulator complex protein PDRO
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6IAA8
  • Protein Name
    Ragulator complex protein LAMTOR1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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IHC image of CSB-PA757083LA01HU diluted at 1:600 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Figure 1. Western blot analysis of LAMTOR1 using anti-LAMTOR1 antibody (A06290-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey COS-7 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LAMTOR1 antigen affinity purified polyclonal antibody (Catalog # A06290-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LAMTOR1 at approximately 18 kDa. The expected band size for LAMTOR1 is at 18 kDa.
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