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Various whole cell extracts (60 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LAMTOR1 antibody (GTX133563) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (60 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LAMTOR1 antibody (GTX133563) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (60 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LAMTOR1 antibody (GTX133563) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

LAMTOR1 antibody

GTX133563
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetLAMTOR1
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Overview

  • Supplier
    GeneTex
  • Product Name
    LAMTOR1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.69 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID55004
  • Target name
    LAMTOR1
  • Target description
    late endosomal/lysosomal adaptor, MAPK and MTOR activator 1
  • Target synonyms
    C11orf59, PDRO, Ragulator1, p18, p27RF-Rho, ragulator complex protein LAMTOR1, RhoA activator C11orf59, late endosomal/lysosomal adaptor and MAPK and MTOR activator 1, lipid raft adaptor protein p18, p27Kip1-releasing factor from RhoA, p27kip1 releasing factor from RhoA, protein associated with DRMs and endosomes, ragulator complex protein PDRO
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6IAA8
  • Protein Name
    Ragulator complex protein LAMTOR1
  • Scientific Description
    Activates RHOA. Binds to CDKN1B and prevents interaction of CDKN1B and RHOA. This leaves RHOA in a form accessible to ARHGEF2, thereby promoting ARHGEF2-mediated exchange of GDP for GTP.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of LAMTOR1 using anti-LAMTOR1 antibody (A06290-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey COS-7 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LAMTOR1 antigen affinity purified polyclonal antibody (Catalog # A06290-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LAMTOR1 at approximately 18 kDa. The expected band size for LAMTOR1 is at 18 kDa.
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