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Immunohistochemical staining of human small intestine shows positivity in plasma.
Immunohistochemical staining of human small intestine shows positivity in plasma.
Immunohistochemical staining of human small intestine shows positivity in plasma.

Anti-LBP Antibody

HPA001508
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetLBP
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-LBP
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3929
  • Target name
    LBP
  • Target description
    lipopolysaccharide binding protein
  • Target synonyms
    BPI fold containing family D, member 2; BPIFD2; lipopolysaccharide-binding protein; LPS-binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP18428
  • Protein Name
    Lipopolysaccharide-binding protein
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LBP using anti-LBP antibody (A00809-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: mouse thymus tissue lysates, Lane 3: HELA whole cell lysates, Lane 4: K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LBP antigen affinity purified polyclonal antibody (Catalog # A00809-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LBP at approximately 65KD. The expected band size for LBP is at 53KD.
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