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LBP Antibody

CSB-PA009787
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetLBP
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Overview

  • Supplier
    Cusabio
  • Product Name
    LBP Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3929
  • Target name
    LBP
  • Target description
    lipopolysaccharide binding protein
  • Target synonyms
    BPI fold containing family D, member 2; BPIFD2; lipopolysaccharide-binding protein; LPS-binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP18428
  • Protein Name
    Lipopolysaccharide-binding protein
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LBP using anti-LBP antibody (A00809-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: mouse thymus tissue lysates, Lane 3: HELA whole cell lysates, Lane 4: K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LBP antigen affinity purified polyclonal antibody (Catalog # A00809-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LBP at approximately 65KD. The expected band size for LBP is at 53KD.
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