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Immunohistochemical staining of human stomach shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human stomach shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human stomach shows moderate cytoplasmic positivity in glandular cells.

Anti-LDAH Antibody

HPA034729
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetLDAH
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-LDAH Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID60526
  • Target name
    LDAH
  • Target description
    lipid droplet associated hydrolase
  • Target synonyms
    C2orf43, hLDAH, lipid droplet-associated hydrolase, UPF0554 protein C2orf43, lipid droplet-associated serine hydrolase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H6V9
  • Protein Name
    Lipid droplet-associated hydrolase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of C2orf43/LDAH using anti-C2orf43/LDAH antibody (A13985). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: monkey COS-7 whole cell lysates, Lane 6: human SK-N-SH whole cell lysates, Lane 7: human SH-SY5Y whole cell lysates, Lane 8: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C2orf43/LDAH antigen affinity purified polyclonal antibody (Catalog # A13985) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C2orf43/LDAH at approximately 37 kDa. The expected band size for C2orf43/LDAH is at 37 kDa.
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Western blot All lanes: LDAH antibody at 8microg/ml Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: A431 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 38, 33, 23 kDa Observed band size: 38 kDa
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