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Immunohistochemical staining of human stomach shows strong cytoplasmic and membranous positivity in chief cells.
Immunohistochemical staining of human stomach shows strong cytoplasmic and membranous positivity in chief cells.
Immunohistochemical staining of human stomach shows strong cytoplasmic and membranous positivity in chief cells.

Anti-LGALS8 Antibody

HPA030491
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetLGALS8
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-LGALS8
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3964
  • Target name
    LGALS8
  • Target description
    galectin 8
  • Target synonyms
    Gal-8, PCTA-1, PCTA1, Po66-CBP, galectin-8, Po66 carbohydrate binding protein, galectin-8g, lectin, galactoside-binding, soluble, 8, po66 carbohydrate-binding protein, prostate carcinoma tumor antigen 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00214
  • Protein Name
    Galectin-8
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of Galectin 8 using anti-Galectin 8 antibody (PB9659). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human COLO-320 whole cell lysates Lane 2: rat testicular tissue lysates Lane 3: mouse Neuro-2A whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Galectin 8 antigen affinity purified polyclonal antibody (Catalog # PB9659) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Galectin 8 at approximately 36KD. The expected band size for Galectin 8 is at 36KD.
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