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Immunohistochemical staining of human cerebellum shows strong nuclear positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows strong nuclear positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows strong nuclear positivity in Purkinje cells.

Anti-LHX1 Antibody

HPA073521
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetLHX1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-LHX1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3975
  • Target name
    LHX1
  • Target description
    LIM homeobox 1
  • Target synonyms
    LIM-1, LIM1, LIM/homeobox protein Lhx1, LIM homeobox protein 1, homeobox protein Lim-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP48742
  • Protein Name
    LIM/homeobox protein Lhx1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of LHX1 using anti-LHX1 antibody (A05816-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LHX1 antigen affinity purified polyclonal antibody (Catalog # A05816-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LHX1 at approximately 43KD. The expected band size for LHX1 is at 43KD.
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