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Immunohistochemical staining of human adrenal gland shows cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human adrenal gland shows cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human adrenal gland shows cytoplasmic positivity in glandular cells.

Anti-LIMS1 Antibody

HPA061230
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetLIMS1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-LIMS1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3987
  • Target name
    LIMS1
  • Target description
    LIM zinc finger domain containing 1
  • Target synonyms
    LIM and senescent cell antigen-like domains 1; LIM and senescent cell antigen-like-containing domain protein 1; LIM-type zinc finger domains 1; particularly interesting new Cys-His protein 1; PINCH; PINCH1; PINCH-1; renal carcinoma antigen NY-REN-48; senescent cell antigen
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP48059
  • Protein Name
    LIM and senescent cell antigen-like-containing domain protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of LIMS1 using anti-LIMS1 antibody (A04072-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIMS1 antigen affinity purified polyclonal antibody (Catalog # A04072-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LIMS1 at approximately 37 kDa. The expected band size for LIMS1 is at 37 kDa.
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