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Western Blot Positive WB detected in: Rat kidney tissue All lanes: LIMS1 antibody at 2.7ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 38, 39, 45, 42 kDa Observed band size: 38 kDa
Western Blot Positive WB detected in: Rat kidney tissue All lanes: LIMS1 antibody at 2.7ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 38, 39, 45, 42 kDa Observed band size: 38 kDa
Western Blot Positive WB detected in: Rat kidney tissue All lanes: LIMS1 antibody at 2.7ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 38, 39, 45, 42 kDa Observed band size: 38 kDa

LIMS1 Antibody

CSB-PA012955EA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetLIMS1
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Overview

  • Supplier
    Cusabio
  • Product Name
    LIMS1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3987
  • Target name
    LIMS1
  • Target description
    LIM zinc finger domain containing 1
  • Target synonyms
    LIM and senescent cell antigen-like domains 1; LIM and senescent cell antigen-like-containing domain protein 1; LIM-type zinc finger domains 1; particularly interesting new Cys-His protein 1; PINCH; PINCH1; PINCH-1; renal carcinoma antigen NY-REN-48; senescent cell antigen
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP48059
  • Protein Name
    LIM and senescent cell antigen-like-containing domain protein 1
  • Scientific Description
    Adapter protein in a cytoplasmic complex linking beta-integrins to the actin cytoskeleton, bridges the complex to cell surface receptor tyrosine kinases and growth factor receptors. Involved in the regulation of cell survival, cell proliferation and cell differentiation.
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of LIMS1 using anti-LIMS1 antibody (A04072-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIMS1 antigen affinity purified polyclonal antibody (Catalog # A04072-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LIMS1 at approximately 37 kDa. The expected band size for LIMS1 is at 37 kDa.
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