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Anti-LIN7C Antibody

ORB2585450
Biorbyt
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetLIN7C
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Overview

  • Supplier
    Biorbyt
  • Product Name
    Anti-LIN7C Antibody
  • Delivery Days Customer
    10
  • Application Supplier Note
    Western blot, 0.25-0.5 microg/ml, Human, Rat Immunohistochemistry, 2-5 microg/ml, Human, Rat Flow Cytometry (Fixed), 1-3 microg/1x106 cells, Human ELISA, 0.1-0.5 microg/ml, -. Adding 0.2 ml of distilled water will yield a concentration of 500 microg/ml
  • Applications
    Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, FC, IHC, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    RPE
  • Gene ID55327
  • Target name
    LIN7C
  • Target description
    lin-7 homolog C, crumbs cell polarity complex component
  • Target synonyms
    LIN-7 protein 3; LIN-7C; LIN-7-C; MALS3; MALS-3; mammalian lin-seven protein 3; protein lin-7 homolog C; VELI3; veli-3; vertebrate lin-7 homolog 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NUP9
  • Protein Name
    Protein lin-7 homolog C
  • Scientific Description
    Anti-LIN7C Antibody. Tested in ELISA, IHC, WB, Flow Cytometry applications. This antibody reacts with Human, Rat.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LIN7C using anti-LIN7C antibody (A07929-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIN7C antigen affinity purified polyclonal antibody (Catalog # A07929-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LIN7C at approximately 22 kDa. The expected band size for LIN7C is at 22 kDa.
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