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Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.

Anti-LIN9 Antibody

HPA030241
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetLIN9
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-LIN9
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID286826
  • Target name
    LIN9
  • Target description
    lin-9 DREAM MuvB core complex component
  • Target synonyms
    BARA; BARPsv; beta subunit-associated regulator of apoptosis; Lin-9; lin-9 homolog; pRB-associated protein; protein lin-9 homolog; rb related pathway actor; TGS; TGS1; TGS2; TUDOR gene similar protein; type I interferon receptor beta chain-associated protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5TKA1
  • Protein Name
    Protein lin-9 homolog
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of LIN9 using anti-LIN9 antibody (A06333-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIN9 antigen affinity purified polyclonal antibody (Catalog # A06333-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LIN9 at approximately 65 kDa. The expected band size for LIN9 is at 62 kDa.
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