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Immunohistochemical staining of human liver shows moderate granular cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows moderate granular cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows moderate granular cytoplasmic positivity in hepatocytes.

Anti-LMAN1 Antibody

HPA002320
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetLMAN1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-LMAN1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3998
  • Target name
    LMAN1
  • Target description
    lectin, mannose binding 1
  • Target synonyms
    ERGIC-53, ERGIC53, F5F8D, FMFD1, MCFD1, MR60, gp58, protein ERGIC-53, ER-Golgi intermediate compartment 53 kDa protein, endoplasmic reticulum-golgi intermediate compartment protein 53, intracellular mannose-specific lectin MR60
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49257
  • Protein Name
    Protein ERGIC-53
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of LMAN1 using anti-LMAN1 antibody (A03628-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LMAN1 antigen affinity purified polyclonal antibody (Catalog # A03628-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LMAN1 at approximately 58 kDa. The expected band size for LMAN1 is at 58 kDa.
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