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Western Blot Positive WB detected in: HepG2 whole cell lysate, Mouse heart tissue All lanes: LMAN1 antibody at 5.3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 58 kDa Observed band size: 58 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, Mouse heart tissue All lanes: LMAN1 antibody at 5.3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 58 kDa Observed band size: 58 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, Mouse heart tissue All lanes: LMAN1 antibody at 5.3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 58 kDa Observed band size: 58 kDa

LMAN1 Antibody

CSB-PA012991LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetLMAN1
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Overview

  • Supplier
    Cusabio
  • Product Name
    LMAN1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3998
  • Target name
    LMAN1
  • Target description
    lectin, mannose binding 1
  • Target synonyms
    ERGIC-53, ERGIC53, F5F8D, FMFD1, MCFD1, MR60, gp58, protein ERGIC-53, ER-Golgi intermediate compartment 53 kDa protein, endoplasmic reticulum-golgi intermediate compartment protein 53, intracellular mannose-specific lectin MR60
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49257
  • Protein Name
    Protein ERGIC-53
  • Scientific Description
    Mannose-specific lectin. May recognize sugar residues of glycoproteins, glycolipids, or glycosylphosphatidyl inositol anchors and may be involved in the sorting or recycling of proteins, lipids, or both. The LMAN1-MCFD2 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of LMAN1 using anti-LMAN1 antibody (A03628-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LMAN1 antigen affinity purified polyclonal antibody (Catalog # A03628-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LMAN1 at approximately 58 kDa. The expected band size for LMAN1 is at 58 kDa.
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