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Immunohistochemical staining of human kidney shows strong membranous and cytoplasmic positivity in tubular cells.
Immunohistochemical staining of human kidney shows strong membranous and cytoplasmic positivity in tubular cells.
Immunohistochemical staining of human kidney shows strong membranous and cytoplasmic positivity in tubular cells.

Anti-LONRF2 Antibody

HPA034595
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetLONRF2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-LONRF2 Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID164832
  • Target name
    LONRF2
  • Target description
    LON peptidase N-terminal domain and ring finger 2
  • Target synonyms
    RNF192, LON peptidase N-terminal domain and RING finger protein 2, RING finger protein 192, neuroblastoma apoptosis-related protease
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ1L5Z9
  • Protein Name
    LON peptidase N-terminal domain and RING finger protein 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of LONRF2 using anti-LONRF2 antibody (A16358-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human A562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LONRF2 antigen affinity purified polyclonal antibody (Catalog # A16358-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LONRF2 at approximately 95 kDa. The expected band size for LONRF2 is at 84 kDa.
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