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LONRF2 Antibody (aa576-625)

LS-C102035
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetLONRF2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    LONRF2 Antibody (aa576-625)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Western Blot: Suggested dilution at 1microg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.
  • Applications
    Western Blot
  • Applications Supplier
    WB (0.2 - 1 µg/ml) Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID164832
  • Target name
    LONRF2
  • Target description
    LON peptidase N-terminal domain and ring finger 2
  • Target synonyms
    LON peptidase N-terminal domain and RING finger protein 2; neuroblastoma apoptosis-related protease; RING finger protein 192; RNF192
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LONRF2 using anti-LONRF2 antibody (A16358-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human A562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LONRF2 antigen affinity purified polyclonal antibody (Catalog # A16358-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LONRF2 at approximately 95 kDa. The expected band size for LONRF2 is at 84 kDa.
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