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Immunohistochemical staining of human duodenum shows moderate cytoplasmic and nuclear positivity in glandular cells.
Immunohistochemical staining of human duodenum shows moderate cytoplasmic and nuclear positivity in glandular cells.
Immunohistochemical staining of human duodenum shows moderate cytoplasmic and nuclear positivity in glandular cells.

Anti-LRRC75A Antibody

HPA028528
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetLRRC75A
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-LRRC75A Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID388341
  • Target name
    LRRC75A
  • Target description
    leucine rich repeat containing 75A
  • Target synonyms
    C17orf76, FAM211A, leucine-rich repeat-containing protein 75A, family with sequence similarity 211, member A, leucine-rich repeat-containing protein C17orf76, leucine-rich repeat-containing protein FAM211A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8NAA5
  • Protein Name
    Leucine-rich repeat-containing protein 75A
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of LRRC75A using anti-LRRC75A antibody (A18461-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRRC75A antigen affinity purified polyclonal antibody (Catalog # A18461-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LRRC75A at approximately 36 kDa. The expected band size for LRRC75A is at 38 kDa.
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