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LRRC75A / C17orf76 Antibody (N-Terminus)

LS-C345938
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetLRRC75A
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    LRRC75A / C17orf76 Antibody (N-Terminus)
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID388341
  • Target name
    LRRC75A
  • Target description
    leucine rich repeat containing 75A
  • Target synonyms
    C17orf76, FAM211A, leucine-rich repeat-containing protein 75A, family with sequence similarity 211, member A, leucine-rich repeat-containing protein C17orf76, leucine-rich repeat-containing protein FAM211A
  • Host
    Rabbit
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LRRC75A using anti-LRRC75A antibody (A18461-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRRC75A antigen affinity purified polyclonal antibody (Catalog # A18461-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LRRC75A at approximately 36 kDa. The expected band size for LRRC75A is at 38 kDa.
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