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Immunohistochemical staining of human testis shows strong positivity in basal cells.
Immunohistochemical staining of human testis shows strong positivity in basal cells.
Immunohistochemical staining of human testis shows strong positivity in basal cells.

Anti-LSM8 Antibody

HPA020116
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetLSM8
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-LSM8 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51691
  • Target name
    LSM8
  • Target description
    LSM8 homolog, U6 small nuclear RNA associated
  • Target synonyms
    NAA38, LSM8 homolog, U6 small nuclear RNA associated, LSM8 U6 small nuclear RNA associated, MAK31-like protein, N-alpha-acetyltransferase 38, NatC auxiliary subunit, U6 snRNA-associated Sm-like protein LSm8
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95777
  • Protein Name
    U6 snRNA-associated Sm-like protein LSm8
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of LSM8 using anti-LSM8 antibody (A10947). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human HEPG2 whole cell lysates, Lane 4: human U937 whole cell lysates, Lane 5: human Jurkat whole cell lysates, Lane 6: human Raji whole cell lysates, Lane 7: human THP-1 whole cell lysates, Lane 8: human K562 whole cell lysates, Lane 9: rat testis tissue lysates, Lane 10: rat lung tissue lysates, Lane 11: rat liver tissue lysates, Lane 12: rat pancreas tissue lysates, Lane 13: mouse testis tissue lysates, Lane 14: mouse lung tissue lysates, Lane 15: mouse liver tissue lysates, Lane 16: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LSM8 antigen affinity purified polyclonal antibody (Catalog # A10947) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LSM8 at approximately 16KD. The expected band size for LSM8 is at 11KD.
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TargetLSM8
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TargetLSM8
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IHC image of CSB-PA013212LA01HU diluted at 1:600 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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TargetLSM8
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