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LSM8 / NAA38 Antibody

LS-C680791
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetLSM8
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    LSM8 / NAA38 Antibody
  • Delivery Days Customer
    14
  • Applications
    ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA, IHC, IHC-P
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID51691
  • Target name
    LSM8
  • Target description
    LSM8 homolog, U6 small nuclear RNA associated
  • Target synonyms
    NAA38, LSM8 homolog, U6 small nuclear RNA associated, LSM8 U6 small nuclear RNA associated, MAK31-like protein, N-alpha-acetyltransferase 38, NatC auxiliary subunit, U6 snRNA-associated Sm-like protein LSm8
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LSM8 using anti-LSM8 antibody (A10947). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human HEPG2 whole cell lysates, Lane 4: human U937 whole cell lysates, Lane 5: human Jurkat whole cell lysates, Lane 6: human Raji whole cell lysates, Lane 7: human THP-1 whole cell lysates, Lane 8: human K562 whole cell lysates, Lane 9: rat testis tissue lysates, Lane 10: rat lung tissue lysates, Lane 11: rat liver tissue lysates, Lane 12: rat pancreas tissue lysates, Lane 13: mouse testis tissue lysates, Lane 14: mouse lung tissue lysates, Lane 15: mouse liver tissue lysates, Lane 16: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LSM8 antigen affinity purified polyclonal antibody (Catalog # A10947) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LSM8 at approximately 16KD. The expected band size for LSM8 is at 11KD.
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