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Immunohistochemical staining of human adrenal gland shows strong nuclear and cytoplasmic positivity in cortical cells.
Immunohistochemical staining of human adrenal gland shows strong nuclear and cytoplasmic positivity in cortical cells.
Immunohistochemical staining of human adrenal gland shows strong nuclear and cytoplasmic positivity in cortical cells.

Anti-LTA4H Antibody

HPA008399
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetLTA4H
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-LTA4H
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4048
  • Target name
    LTA4H
  • Target description
    leukotriene A4 hydrolase
  • Target synonyms
    leukotriene A-4 hydrolase; LTA-4 hydrolase; testicular secretory protein Li 27; tripeptide aminopeptidase LTA4H
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09960
  • Protein Name
    Leukotriene A-4 hydrolase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: 293 whole cell lysate All lanes: LTA4H antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 70, 60, 58, 67 kDa Observed band size: 70 kDa
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Figure 1. Western blot analysis of LTA4H using anti-LTA4H antibody (A02399-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human PC-3 whole cell lysates, Lane 7: rat thymus tissue lysates, Lane 8: rat C6 whole cell lysates, Lane 9: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LTA4H antigen affinity purified polyclonal antibody (Catalog # A02399-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LTA4H at approximately 69 kDa. The expected band size for LTA4H is at 69 kDa.
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